nested pcr中文是什么意思

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中文翻譯
造句

巢式pcr
嵌套式聚合酶鏈式反應

nest n. 1.巢；窩；窟,穴。 2.安息處,休息處；住處,家 ...
pcr PCR ＝polymerase chain reacti ...
rt-nested pcr 逆轉錄-套式聚合酶鏈反應
nested 巢狀的; 內裝的; 嵌套的; 相互套入的
as-pcr 等位基因特異性多聚酶鏈反應
pcr PCR ＝polymerase chain reaction 【生物化學】聚合酶聯鎖反應〔此項技術可用以復制犯罪現場發現的DNA小樣，從而有助于破案〕。
nested barges 套裝駁
nested binding 嵌套結合
nested block 嵌套分程序; 嵌套塊; 嵌套循環
nested blocks 嵌套塊
nested boats 套放救生艇
nested caldera 巢狀破火山口
nested cell 嵌套單元
nested class 巢狀類別嵌套類; 有關
nested classification 抱窩型分類
nested code 嵌套碼
nested conditions 嵌套條件
nested construct 嵌套構造
nested crater 巢狀火山口
nested craters 巢狀火山口
nested data 數據套
nested dc 多套
nested decision 嵌套判定
nested deletion 二十四碳單烯酸; 嵌套缺失
nested deposit 巢狀礦床

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例句與用法

Mycoplasma detection methods - part 3 : nested pcr assay
支原體檢測方法.第3部分:窩形pcr檢驗法
Rapid detection of listeria monocytogenes in food with multiplex - nested pcr
檢測食品中單增李斯特菌研究
Study of the nested pcr detection salmonella typhi in the blood specimens
巢式聚合酶鏈反應快速檢測血液標本中
Definition of nested pcr
巢式pcr的定義
By using the method of genome walking , two fragments were cloned from genome walker library after the semi - nested pcr . the result of sequencing the bigger fragment showed that this 706bp sequence contained the atg start codon
通過半巢式的基因步移法，經二輪pcr后，從genomewalker文庫分離到2個片段，較大的片段測序表明該706bp序列含有編碼ast的起始密碼子atg ，其上游序列為啟動子序列。
Using nested pcr and draft human genome searching , we successfully cloned the tsarg2 and mouse homeobox gene and made rudiment study on its functions . there were three parts in the study , and the following is its main experimental methods and results
本研究利用巢式pcr技術和人類基因組草圖搜索法，從上述est出發，于2000年6月成功地克隆了人類tsarg2基因及小鼠同源基因srg2 ，并對其功能進行了初步研究。
According to the amino acid sequence resulted from our previous research about tb22kda protein and the related literatures about gene sequence of allergenic protein in common buckwheat , we designed primers and got the structure gene successfully . by 3 ' - race method combined with nested pcr , the 3 ' - end nuclear acid sequence was also obtained ; in additon , for the 5 ' - end sequence , we selected a specific conserved nuclear acid sequence as the 5 ' primer and part of structure gene sequence as the 3 " primer , and till now , partial 5 ' - end sequence has been amplified as well
本研究根據先前分離純化所得天然tb22kda蛋白經maldi - tof - ms (質譜法)測得的氨基酸序列和文獻報道的過敏蛋白核苷酸序列設計引物，擴增克隆了該過敏蛋白結構基因的編碼序列；根據測得的序列設計特異性引物，并利用3 ’ - race方法結合巢式pcr擴增得到基因的3 ’末端；依據同源性比較的結果選用一段保守序列為5 ’引物，并根據結構基因內部序列設計3 ’特異性引物，進一步獲得了該基因5 ’端的部分序列。
The gene cloning and sequence analysis of senv - d and senv - h isolated from china according to the published nucleotide sequences of sen viruses , specific primers were designed and synthesized . from the serum of two chinese patients with non - a - e hepatitis , one senv - d isolate named senv - d - bj1 spanning the complete coding region was amplified by semi - nested pcr , another isolate named senv - d - bj2 spanning the partial coding region ( including orf1 and orf2 ) was amplified too . from one blood donor serum , two senv - h isolates named senv - h12 - 1 and senv - h 12 - 2 spanning the complete coding region were amplified by nested pcr respectively
Sen病毒d和h亞型中國分離株的克隆及序列分析我們在前期工作的基礎上，結合已發表的文章及基因序列，針對senv - d和senv - h基因組設計特異性引物，利用套式pcr技術從兩例non - a - e肝炎患者血清中分段克隆得到了一個senv - d亞型分離株( senv - d - bj1 )的全部編碼區基因序列，還得到了一個senv - d亞型分離株( senv - d - bj2 )的大部分編碼區基因序列(包括orf1和orf2 ) ；從一例健康人血清中分段克隆得到了兩個senv - h亞型分離株( senv - h12 - 1和senv - h12 - 2 )的全部編碼區基因序列。
Firstly , the eo genes of csfv - jl and csfv - ln9912 strains were amplified by rt - pcr and nested pcr and then sequenced after cloned into t easy vector . comparing the eo sequences with other strains eo by biosoftwares , dnastar5 . 0 and bioedit , the phylogenetic analyse revealed that all of the strains we have sequenced could be divided into groupl and groupii . two amino acid streches of 15 csfv strains eo , which form the rnase active site , and histidine residues essential for rnase catalysis in both ones were highly conserved . the eo protein propertys of antigen epitope , hydrophobicity , isoelectric point were also predicted by bioinformatic method
首先，采用rt - pcr和nest - pcr技術擴增了csfv - jl株和csfv - ln9912株eo基因，插入teasy載體后測序，應用dnastar5 . 0和bioedit軟件將其與本實驗室已測其它毒株和genebank中登錄的csfv代表毒株eo基因序列進行比較，繪制了遺傳進化樹并預測了eo蛋白的抗原表位、疏水性、等電點等特性。
Pbluebachisc - vp7 was identified and analped by compnding restriction endonuclease , pcr and nested pcr on the basis of the genetic sites of pbluebachisc , which was idenhfied as vp7 gene of prv hafied pbluebachisc - vp7 and barmidn - blue dna were cbeinfected insect cell sro and harvested mmbinan beculovirus 5d later identification with restriction empme and pcr showed vp7 gene has been arembwt comehy with baculovirus dna and namd a - l
純化該重組質粒并與線性桿狀病毒dnabac - n - blue共轉染昆蟲細胞sr9 ， 5d后收獲重組病毒。重組桿狀病毒dna分子的pcr及酶切鑒定表明，獲得了prv - vp7基因與桿狀病毒dna的重組子，命名為a - 1代病毒。